Protection of mice against an influenza virus infection by oral vaccination with viral nucleoprotein incorporated into immunostimulating complexes
Identifieur interne : 001E24 ( Main/Exploration ); précédent : 001E23; suivant : 001E25Protection of mice against an influenza virus infection by oral vaccination with viral nucleoprotein incorporated into immunostimulating complexes
Auteurs : K. Scheepers [Allemagne] ; H. Becht [Allemagne]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 1994-11-01.
English descriptors
- Teeft :
- Antibody, Antibody assay, Antigenic structure, Bacterial lipopolysaccharide, Blood serum, Body weight, Bovine serum albumin, Bronchial lavage, Bronchoalveolar, Bronchoalveolar fluid, Challenge infection, Chorioallantoic membranes, Crucial role, Cytokine, Cytotoxic, Cytotoxic effect, Days post infection, Drinking water, Embryonated chicken eggs, Experimental animals, Experimental conditions, Gastrointestinal tract, High antibody titers, High levels, High titers, Host cells, Hyperimmune rabbit serum, Immune response, Immunization, Immunol, Immunostimulating complexes, Incubation period, Influenza, Influenza cells, Influenza cytotoxic clones, Influenza vaccine, Influenza virus, Influenza virus nucleoprotein, Influenza viruses, Intragastric, Intragastric administration, Intragastric dose, Iscom, Iscom immunization, Iscom particles, Iscoms, Last antigen application, Last immunization, Lethal challenge, Lethal challenge infection, Lethal infection, Lipid, Lipid stock solution, Lipophilic anchor, Lung tissue, Lymphocyte, Microbiol immunol, Molecular weight markers, Monoclonal antibodies, Mouse, Mowat amci, Mucous membrane, Nucleoprotein, Nude mice, Observation period, Oral administration, Oral cavity, Oral immunization, Oral vaccination, Other side, Other studies, Partial protection, Peroral immunization, Protective effect, Protective immunity, Pulmonary lesions, Pulmonary pathology, Pulmonary tissue, Reaction mixture, Recombinant, Recombinant nucleoprotein, Recombinant vaccinia virus, Room temperature, Scand, Serial passages, Sodium bicarbonate, Spleen lymphocytes, Stitz, Subcutaneous, Subcutaneous immunization, Subcutaneous injection, Such effect, Target cells, Toxic effect, Unprotected groups, Untreated controls, Vaccinated, Vaccinated animals, Vaccination, Vaccine, Vaccinia, Vaccinia virus, Viral, Viral antigen, Viral nucleoprotein, Virus, Virus infection, Virus nucleoprotein, Virus replication, Whole observation period.
Abstract
Abstract: Influenza A virus nucleoprotein (NP) was integrated into immunostimulating complexes (ISCOMs) after attachment of bacterial lipopolysaccharide to the antigen. Oral immunization with these NP-ISCOMs protected mice fully against an otherwise lethal challenge infection with an unrelated influenza virus subtype without the appearance of severe clinical signs or extensive pathological lesions in the lungs. Mice immunized with analogous bovine serum albumine-incorporated ISCOMs all died. After oral immunization, high titers of NP-specific antibodies, particularly IgA, could be detected in the bronchoalveolar fluid and in the blood serum. No cytotoxic lymphocytes could be demonstrated in the spleens or the lungs of vaccinated mice, and no anti-NP antibody-dependent cytolysis of infected host cells was mediated by complement or in the form of an antibody-dependent cell cytotoxicity. However, a vigorous delayed-type hypersensitivity reaction was produced after probing vaccinated animals with purified NP. No comparable protective immunity or antibody response was induced by a strictly intragastric administration of NP-ISCOMs. It appears, therefore, that the general and local immune response in the lungs was primarily stimulated through contact of NP-ISCOMs with the mucous membrane of the oro-pharyngeal cavity and that cytotoxic effects did not play a major role for the establishment of the protective immunity. Partial protection against a lethal challenge was observed in chickens immunized with NP-ISCOMs in the drinking water.
Url:
DOI: 10.1007/BF00198460
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000551
- to stream Istex, to step Curation: 000551
- to stream Istex, to step Checkpoint: 000B95
- to stream Main, to step Merge: 001F06
- to stream Main, to step Curation: 001E24
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protection of mice against an influenza virus infection by oral vaccination with viral nucleoprotein incorporated into immunostimulating complexes</title><author><name sortKey="Scheepers, K" sort="Scheepers, K" uniqKey="Scheepers K" first="K." last="Scheepers">K. Scheepers</name></author><author><name sortKey="Becht, H" sort="Becht, H" uniqKey="Becht H" first="H." last="Becht">H. Becht</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1AC50F4079D4F0F035EF6A376111722970587B1A</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1007/BF00198460</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-DFLCWMKT-8/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000551</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000551</idno><idno type="wicri:Area/Istex/Curation">000551</idno><idno type="wicri:Area/Istex/Checkpoint">000B95</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B95</idno><idno type="wicri:doubleKey">0300-8584:1994:Scheepers K:protection:of:mice</idno><idno type="wicri:Area/Main/Merge">001F06</idno><idno type="wicri:Area/Main/Curation">001E24</idno><idno type="wicri:Area/Main/Exploration">001E24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Protection of mice against an influenza virus infection by oral vaccination with viral nucleoprotein incorporated into immunostimulating complexes</title><author><name sortKey="Scheepers, K" sort="Scheepers, K" uniqKey="Scheepers K" first="K." last="Scheepers">K. Scheepers</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Virologie, Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, D-35392, Giessen</wicri:regionArea><wicri:noRegion>35392, Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Becht, H" sort="Becht, H" uniqKey="Becht H" first="H." last="Becht">H. Becht</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Virologie, Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, D-35392, Giessen</wicri:regionArea><wicri:noRegion>35392, Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="ISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1994-11-01">1994-11-01</date><biblScope unit="volume">183</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="278">278</biblScope></imprint><idno type="ISSN">0300-8584</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8584</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antibody</term><term>Antibody assay</term><term>Antigenic structure</term><term>Bacterial lipopolysaccharide</term><term>Blood serum</term><term>Body weight</term><term>Bovine serum albumin</term><term>Bronchial lavage</term><term>Bronchoalveolar</term><term>Bronchoalveolar fluid</term><term>Challenge infection</term><term>Chorioallantoic membranes</term><term>Crucial role</term><term>Cytokine</term><term>Cytotoxic</term><term>Cytotoxic effect</term><term>Days post infection</term><term>Drinking water</term><term>Embryonated chicken eggs</term><term>Experimental animals</term><term>Experimental conditions</term><term>Gastrointestinal tract</term><term>High antibody titers</term><term>High levels</term><term>High titers</term><term>Host cells</term><term>Hyperimmune rabbit serum</term><term>Immune response</term><term>Immunization</term><term>Immunol</term><term>Immunostimulating complexes</term><term>Incubation period</term><term>Influenza</term><term>Influenza cells</term><term>Influenza cytotoxic clones</term><term>Influenza vaccine</term><term>Influenza virus</term><term>Influenza virus nucleoprotein</term><term>Influenza viruses</term><term>Intragastric</term><term>Intragastric administration</term><term>Intragastric dose</term><term>Iscom</term><term>Iscom immunization</term><term>Iscom particles</term><term>Iscoms</term><term>Last antigen application</term><term>Last immunization</term><term>Lethal challenge</term><term>Lethal challenge infection</term><term>Lethal infection</term><term>Lipid</term><term>Lipid stock solution</term><term>Lipophilic anchor</term><term>Lung tissue</term><term>Lymphocyte</term><term>Microbiol immunol</term><term>Molecular weight markers</term><term>Monoclonal antibodies</term><term>Mouse</term><term>Mowat amci</term><term>Mucous membrane</term><term>Nucleoprotein</term><term>Nude mice</term><term>Observation period</term><term>Oral administration</term><term>Oral cavity</term><term>Oral immunization</term><term>Oral vaccination</term><term>Other side</term><term>Other studies</term><term>Partial protection</term><term>Peroral immunization</term><term>Protective effect</term><term>Protective immunity</term><term>Pulmonary lesions</term><term>Pulmonary pathology</term><term>Pulmonary tissue</term><term>Reaction mixture</term><term>Recombinant</term><term>Recombinant nucleoprotein</term><term>Recombinant vaccinia virus</term><term>Room temperature</term><term>Scand</term><term>Serial passages</term><term>Sodium bicarbonate</term><term>Spleen lymphocytes</term><term>Stitz</term><term>Subcutaneous</term><term>Subcutaneous immunization</term><term>Subcutaneous injection</term><term>Such effect</term><term>Target cells</term><term>Toxic effect</term><term>Unprotected groups</term><term>Untreated controls</term><term>Vaccinated</term><term>Vaccinated animals</term><term>Vaccination</term><term>Vaccine</term><term>Vaccinia</term><term>Vaccinia virus</term><term>Viral</term><term>Viral antigen</term><term>Viral nucleoprotein</term><term>Virus</term><term>Virus infection</term><term>Virus nucleoprotein</term><term>Virus replication</term><term>Whole observation period</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Influenza A virus nucleoprotein (NP) was integrated into immunostimulating complexes (ISCOMs) after attachment of bacterial lipopolysaccharide to the antigen. Oral immunization with these NP-ISCOMs protected mice fully against an otherwise lethal challenge infection with an unrelated influenza virus subtype without the appearance of severe clinical signs or extensive pathological lesions in the lungs. Mice immunized with analogous bovine serum albumine-incorporated ISCOMs all died. After oral immunization, high titers of NP-specific antibodies, particularly IgA, could be detected in the bronchoalveolar fluid and in the blood serum. No cytotoxic lymphocytes could be demonstrated in the spleens or the lungs of vaccinated mice, and no anti-NP antibody-dependent cytolysis of infected host cells was mediated by complement or in the form of an antibody-dependent cell cytotoxicity. However, a vigorous delayed-type hypersensitivity reaction was produced after probing vaccinated animals with purified NP. No comparable protective immunity or antibody response was induced by a strictly intragastric administration of NP-ISCOMs. It appears, therefore, that the general and local immune response in the lungs was primarily stimulated through contact of NP-ISCOMs with the mucous membrane of the oro-pharyngeal cavity and that cytotoxic effects did not play a major role for the establishment of the protective immunity. Partial protection against a lethal challenge was observed in chickens immunized with NP-ISCOMs in the drinking water.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Scheepers, K" sort="Scheepers, K" uniqKey="Scheepers K" first="K." last="Scheepers">K. Scheepers</name></noRegion><name sortKey="Becht, H" sort="Becht, H" uniqKey="Becht H" first="H." last="Becht">H. Becht</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E24 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E24 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:1AC50F4079D4F0F035EF6A376111722970587B1A
|texte= Protection of mice against an influenza virus infection by oral vaccination with viral nucleoprotein incorporated into immunostimulating complexes
}}
| This area was generated with Dilib version V0.6.33. |  |